Heart Rhythm Research at UCSF: Junior Specialist
Company: University of California - San Francisco
Location: San Francisco
Posted on: November 11, 2019
HEART RHYTHM RESEARCH AT UCSF: JUNIOR SPECIALIST APPLY NOW TO HEART
RHYTHM RESEARCH AT UCSF: JUNIOR SPECIALIST
* SCHOOL OF MEDICINE - Medicine
Open date: October 30th, 2019
Next review date: Thursday, Nov 14, 2019 at 11:59pm (Pacific
Apply by this date to ensure full consideration by the committee.
Final date: Friday, Apr 30, 2021 at 11:59pm (Pacific Time)
Applications will continue to be accepted until this date, but
those received after the review date will only be considered if the
position has not yet been filled.
Heart Rhythm Research at UCSF: Junior Specialist
The incumbent will be involved in performing laboratory experiments
and physiological analysis for a basic research program led by Dr.
Vasanth Vedantham, a clinical cardiac electrophysiologist and basic
researcher in the Division of Cardiology, University of California,
San Francisco. The research will be focused on molecular biology
and physiology of the heart under normal conditions, under
physiological stress, and in pathological conditions in mouse
models with special attention to heart rhythm.
BRIEF JOB OVERVIEW: Under the general direction of Dr. Vasanth
Vedantham, the incumbent will perform scientific research
experiments related to various research projects for the
supervisors research program. These include both in vitro and in
vivo experiments relating to heart rhythm. The individual's
responsibilities will include: independently performing laboratory
research experiments, performing mouse surgeries to implant
transmitters, managing a mouse colony, imaging using confocal and
light sheet microsroscopy, and preparation of libraries for single
cell sequencing technologies, and general lab maintenance. Although
the candidate will have her or his own dedicated research project,
s(he) will also assist other lab members in lab maintenance duties
including PCR genotyping, mouse colony maintenance, and assisting
the principal investigator and lab manager in all laboratory
matters when needed.
QUALIFICATIONS: The individual should hold a bachelor s degree and
have at least 1 year of experience (part-time acceptable) in the
following areas: cardiovascular research, mouse handling and
euthanasia, mouse surgery, EKG analysis in mouse models, and basic
SPECIFIC JOB DESCRIPTION:
Performing laboratory experiments (40%)
The individual will work very closely with the principal
investigator and the lab manager to perform and to ensure
completion of research experiments related to the research projects
of the laboratory. The individual will set up and perform novel
experiments and molecular-cellular techniques necessary for
research projects of the lab such as molecular cloning, DNA/RNA
isolation, mouse heart dissection, mouse cardiomyocyte isolation,
mouse surgery to implant transmitters, tissue processing and
handling of biological specimen for histological studies including:
fixation (small and large tissue, and cultured cells), embedding
(paraffin, plastic and frozen), sectioning (paraffin, plastic and
frozen), and histological staining with antibodies. The individual
must perform microscopy (conventional immunofluorescence, confocal,
and light sheet) of fixed cells, tissue sections, mouse embryos,
and mouse hearts. The individual will perform basic molecular and
biochemical assays for RNA/DNA detection using PCR, RT-PCR and
Western Blotting, isolation of RNA/DNA, and protein from cells and
animal tissue, ELISA, and cloning. The individual may also perform
cell culture experiments involving transfection and analysis of
reporter gene activity and promoter activity in cells.
Physiological and Imaging Data Analysis, and Writing (40%)
A major part of the lab s effort involves analysis of physiological
data sets obtained with implantable transmitters and other types of
physiological studies in mice. Specific types of analysis might
include defining electrocardiographic intervals, identification of
arrhythmias from EKG data, higher-order analysis including heart
rate variability and power spectra. In addition, 3D reconstruction
and quantification of microscopy images including those from whole
mount light sheet microscopy as well as confocal microscopy of
tissue sections will be required. The incumbent will also be
responsible for preparing figures and manuscripts for publication
or presentation at meetings.
Lab management (20%)
* The individual will assist the Supervisor and Lab Manager as
needed to ensure, in a timely manner and in compliance with all
UCSF and regulatory agency policies and rules, the: completion of
all personnel training and certifications; coordination of various
lab inspections (e.g. OS&H, IACUC); and renewals, modifications
and new applications of IACUC/OS&H protocols. The incumbent
will ensure that chemical, controlled, and expired substances
inventory is performed as required.
* Performing lab maintenance tasks along with others in the lab at
the direction of the lab manager. Examples might be having primary
responsibility for maintaining a particular instrument or keeping a
particular area of the lab clean, well stocked with consumable
supplies, and in order.
* Assisting other lab members with PCR genotyping of mouse and
* Maintaining a mouse colony and assisting others as needed.
UC San Francisco seeks candidates whose experience, teaching,
research, or community service has prepared them to contribute to
our commitment to diversity and excellence.
The University of California is an Equal Opportunity/Affirmative
Action Employer. All qualified applicants will receive
consideration for employment without regard to race, color,
religion, sex, sexual orientation, gender identity, national
origin, disability, age or protected veteran status.
Please apply online at https://aprecruit.ucsf.edu/JPF02746
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