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Research Technologist - Stem Cell Core

Company: Gladstone Institutes
Location: San Francisco
Posted on: September 24, 2022

Job Description:

Category:ScienceLab/Area:Stem Cell CoreDescription:The Stem Cell Core is a state-of-the-art facility providing comprehensive pluripotent stem cell related services (standard and customized), training and consultations. Our focus includes reprogramming of somatic cells into iPSCs, differentiation of iPSCs into various cell lineages and genome editing in iPSCs. Our mission is to support academic and industry sector stem cell researchers to accelerate cures through stem cell research. We are currently recruiting for a highly motivated biologist to join our Stem Cell Core Laboratory as a Research Technologist. This is a laboratory-based position, and expertise in tissue culture and molecular biology is highly preferred.Responsibilities

  • Culture human iPSCs, primary fibroblasts and peripheral blood mononuclear cells (PBMCs)
  • Characterize human iPSCs to assess their quality by visual inspection and molecular and biochemical techniques (immunocytochemistry, flow cytometry, RT-qPCR)
  • Perform quarterly mycoplasma testing on cell lines and report results to users
  • Human iPSC reprogramming from fibroblasts or PBMCs
  • Differentiation of human iPSCs into different cell types using established protocols with optimizations
  • CRISPR gene editing of human iPSCs
    • Manage and operate Core facility semi-independently
    • Maintain a detailed lab notebook
    • Help manage collaborative projects with leading Gladstone & UCSF stem cell labs
    • Work collaboratively and cohesively in a small, highly-functional group
    • Report to Core Director regularly to discuss on-going projects and infrastructure issues
    • Interact and communicate effectively with many multinational researchers on a weekly basis
    • Enforce Core rules, procedures and guidelines
    • Communicate with vendors to maintain professional relationships and negotiate discounted pricing on equipment, reagents and service
    • Provide researchers with technical advice
    • This is a full-time position. Availability for limited work on weekends is required.The ideal candidate will be motivated to facilitate cutting-edge disease-related research. The objective for the person in this position will be to assist investigators to attain ambitious research goals by providing scientific expertise, services, and maintenance of an orderly, organized environment where resources can be most effectively accessed and utilized by a large number of researchers.Qualifications
      • B.S. Biological Sciences, or equivalent
      • 2+ years of work experience in a laboratory applying molecular biology and cell culture techniques
      • Experience in pluripotent human stem cell culture, propagation and differentiation preferred but not required
      • Must have experience working with scientists to provide research support and laboratory management
      • Good understanding of molecular and cellular biology principles required
      • Enthusiastic team player
      • Ability to problem-solve and provide solutions
      • Ability to be resourceful and proactive to assisting researchers and carrying out experiments
      • Manage time appropriately in an environment requiring attention to shifting demands
      • Accept constructive feedback to implement operational modifications where necessary to improve accuracy, efficiency, and cost-effectiveness
      • Desire to learn new scientific techniquesGladstone Perks & Benefits:
        • People-work with talented, committed, and supportive teammates within an organization that values each member of its community.
        • A meaningful place to grow and learn-whether it's your professional skills or scientific knowledge, we have the resources and environment to advance either so you can better support Gladstone's mission to drive a new era of discovery in disease-oriented science and to mentor tomorrow's leaders in an inspiring and diverse environment.
        • Healthy work/life balance-you are highly engaged and productive at work because you can have time to recharge and enjoy a vibrant life outside of work.
        • Compensation-competitive salary and paid vacation.
        • Excellent benefits-generous medical, dental, vision, retirement plan, and commuter benefits; access to free shuttle transportation.Additional Information:
          • Title and salary will be commensurate with education and experience.
          • Gladstone is an equal opportunity employer.

Keywords: Gladstone Institutes, San Francisco , Research Technologist - Stem Cell Core, Other , San Francisco, California

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