Laboratory Associate - Protein Production
Company: Mammoth Biosciences
Location: Brisbane
Posted on: May 17, 2022
Job Description:
OPPORTUNITYMammoth is seeking a Laboratory Associate with
protein production expertise to join its growing Discovery team.
The Discovery team is responsible for developing novel
CRISPR-associated (Cas) nucleases for genome editing applications.
The successful candidate will be responsible for executing
laboratory workflows to produce Cas nucleases for genome editing.
The primary focus for this role will be on protein production and
process development. This is an excellent development opportunity
for researchers who want to learn new skills and start their career
working on cutting-edge CRISPR technologies.KEY
RESPONSIBILITIES
- Execute internal microbial protein production for research
applications
- Contribute to process development to establish methods to
produce proteins and protein complexes for biochemical
characterization and structural interrogation
- Rigorously document newly developed methods and results
- Provide regular updates at internal meetings
- Other duties as assignedREQUIRED QUALIFICATIONS
- Bachelor's degree in molecular biology, biochemistry, or a
related field
- 2+ years of laboratory experience in academia or industry
developing and executing bacterial protein expression workflows
including, but not limited to:
- Bacterial transformation and growth in liquid media
- Execution of upstream (cell harvest, lysis, clarification) and
downstream (liquid chromatography, filtration, concentration)
processes
- Protein analytical methods (Eg. homogeneity evaluation, protein
quantitation, detection / quantitation of process-related
impurities such as host-cell protein, endotoxin, nucleic
acids)
- Generation of working cell banks
- FPLC systems (Eg. Cytiva AKTA, BioRad NGC) and associated
software packagesPREFERRED QUALIFICATIONS
- Prior experience executing upstream and / or downstream process
development
- Previous protein manufacturing experience (research-grade or
cGMP)
- Hands-on experience in the following areas:
- Biochemical assays
- Molecular biology (PCR, DNA/RNA purification and
quantitation)
- Computer and scripting skills (Benchling, Geneious, JMP, SQL,
R, python)BENEFITS
- Company-paid health/vision/dental benefits
- Unlimited vacation and generous sick time
- Company-sponsored meals and snacks
- Wellness, caregiver and ergonomics benefits
- 401(k) with company matchingMammoth Biosciences is harnessing
the diversity of life to power the next generation of CRISPR
products. Through the discovery and engineering of novel CRISPR
systems, the company is enabling the full potential of its platform
to read and write the code of life. Mammoth aims to develop
permanent genetic cures through best-in-class in vivo and ex vivo
therapies and to democratize disease detection with on-demand
diagnostics. Mammoth can provide enhanced diagnostics and genome
editing for life science research, healthcare, agriculture,
biodefense and more. Based in the San Francisco Bay Area, Mammoth
Biosciences is co-founded by CRISPR pioneer and Nobel Laureate
Jennifer Doudna and Trevor Martin, Janice Chen, and Lucas
Harrington. The firm is backed by top institutional investors
including Redmile Group, Foresite Capital, Senator Investment
Group, Sixth Street, Decheng, Mayfield, NFX, and 8VC, along with
leading individual investors including Brook Byers, Tim Cook, and
Jeff Huber.TEAMOur small-size proteins, such as Cas14 and CasPhi,
are poised to transform disease treatment enabling novel editing
approaches and delivery options that are difficult to achieve or
not even possible with previously described CRISPR-Cas systems. Our
teams are responsible for discovering and engineering novel CRISPR
systems, establishing CRISPR-based diagnostics, and translating our
unique CRISPR technology into therapeutic treatments with the
ultimate goal of cures for indications with high medical need.It is
our policy and intent to provide equal opportunity to all persons
without regard to race, color, religion, political affiliation,
sex/gender (including gender expression/identity, pregnancy,
childbirth and related medical conditions), marital status,
registered domestic partner status, sexual orientation, age,
ancestry, national origin, veteran status, disability, medical
condition, genetic characteristics, and/or any other basis
protected by law. This policy covers all facets of employment
including, but not limited to: recruitment, selection, placement,
promotions, transfers, demotions, terminations, training, and
compensation.
Keywords: Mammoth Biosciences, San Francisco , Laboratory Associate - Protein Production, Other , Brisbane, California
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